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2 edition of In situ bioremediation of trichloroethylene using Burkholderia cepacia G4 PR1 found in the catalog.

In situ bioremediation of trichloroethylene using Burkholderia cepacia G4 PR1

Richard A. Snyder

In situ bioremediation of trichloroethylene using Burkholderia cepacia G4 PR1

analysis of microbial ecology parameters for risk assessment

by Richard A. Snyder

  • 150 Want to read
  • 35 Currently reading

Published by U.S. Environmental Protection Agency, Research and Development, National Health and Environmental Effects Research Laboratory in Gulf Breeze, FL .
Written in English

    Subjects:
  • Groundwater -- Purification,
  • Groundwater -- Pollution,
  • Microbial ecology,
  • Trichloroethylene -- Environmental aspects

  • Edition Notes

    Other titlesAnalysis of microbial ecology parameters for risk assessment
    StatementRichard A. Snyder
    SeriesEnvironmental research brief
    ContributionsNational Health and Environmental Effects Research Laboratory (U.S.)
    The Physical Object
    Pagination7 p. :
    ID Numbers
    Open LibraryOL15229458M

    B. cepacia complex bacteria as biocontrol agents B. cepacia complex bacteria are naturally abundant in soil, water, and on plant surfaces (4). They are distinctive in their ability to metabolize a broad range of organic compounds as carbon and energy sources, an attribute which has spurred the development of their use in bioremediation of soil and groundwater contaminated with chlorinated. Shields et al. [53], working on a strain of B. cepacia G4 (PR), found it to constitutively express a toluene catabolic pathway by a toluene ortho-monooxygenase. Johnson and Olsen [54] cloned and sequenced the genes Strategies for the aerobic co-metabolism of chlorinated solvents Semprini for the toluene 2-monooxygenase enzyme by:

    @article{osti_, title = {Bioremediation of Trichloroethylene-Contaminated Sediments Augmented with a Dehalococcoides Consortia}, author = {McKinsey, P C}, abstractNote = {At the Department of Energy's (DOE) Savannah River Site (SRS) in Aiken, SC there are a number of sites contaminated with Chlorinated Ethenes (CE) due to past disposal practices.   Appl Environ Microbiol –14 ethylene by Burkholderia cepacia G4 in a two-stage con- Pant P, Pant S () A review: advances in microbial remediation tinuous stirred tank reactor/trickling biofilter system. of trichloroethylene (TCE).

    KR1 and Burkholderia cepacia G4 PR1 are able to use phenol, but not dichlorophenol, as a sole carbon and energy source. Similarly, the toluene o-monooxygenase (Tom) of B. cepacia G4 PR1 cometabolically degrades trichloroeth-ylene (Nelson et al., ) to CO2 and Cl −, but is unable to catalyze degradation of the more chlorinated PCE. Because. B. cepacia G4 (Folsom and Chapman, ) and B. higher total surface area than SIRANe. cepacia PR1 23 (TOM 23C; Shields and Reagin,) were The bioreactor was operated continuously with re-provided by Professor Reagin at the University of West spect to both the nutrient and TCE flow rates. The Florida. G4 is the parent strain of PR1.


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In situ bioremediation of trichloroethylene using Burkholderia cepacia G4 PR1 by Richard A. Snyder Download PDF EPUB FB2

In situ bioremediation of trichloroethylene using Burkholderia cepacia G4 PR1: analysis of microbial ecology parameters for risk assessment Author: Richard A Snyder ; National Health and Environmental Effects Research Laboratory (U.S.).

Get this from a library. In-situ bioremediation of trichloroethylene using Burkholderia cepacia G4 PR1: analysis of transport parameters for risk assessment. [J R Lawrence; M J Hendry; National Health and Environmental Effects Research Laboratory (U.S.)]. The introduction of bacteria into aquifers for bioremediation purposes requires monitoring of the persistence and activity of microbial populations for efficacy and risk assessment purposes.

Burkholderia cepacia G4 PR1 constitutively expresses a toluene ortho-monooxygenase (tom) that aerobically mineralizes TCE. The experiments used three bacteria, Kleb- siella oxytoca, Burkholderia cepacia G4 PR1, and Pseudomonas #5, a subsurface isolate.

The modeling results suggested that irreversible sorption (kirr) was a function of mean transit time (t0) whereby the product (t0-kirr), which is defined as the equivalent irreversible sorption parameter (A), was constant (mean value of ) at the scales of this investigation.

The experiments used three bacteria, Klebsiella oxytoca, Burkholderia cepacia G4 PR1, and Pseudomonas #5, a subsurface isolate. The modeling results suggested that irreversible sorption (kirr) was a function of mean transit time (to) whereby the product (), which is defined as the equivalent irreversible sorption parameter (A), was constant (mean value of ) at the scales of this investigation.

In situ bioremediation of this contaminant may be possible by the aerobic microorganism Burkholderia cepacia G4. cepacla G4 PR and PR constitutively express a toluene ortftomonooxygenase (torn) due to a secondary transposition of Th5 sequence into a TCE degradative plasmid (TOM) (Nelson etal., ; Shields etal., ).

Phenol-fed microcosms augmented with either B. cepacia strain G4 or PR transformed twice as much TCE as the nonaugmented phenol-fed microcosm.

In addition, should primary substrate addition be a regulatory concern, TCE degradation was observed without primary substrate addition through bioaugmentation using organisms expressing the TCE-transforming enzyme.

Burkholderia cepacia G4 PR1 is a Tn5 insertion mutant which constitutively expresses a toluene ortho-monooxygenase that degrades trichloroethylene (TCE). This ability of G4 PR1 to degrade TCE without aromatic induction may be useful for bioremediation of TCE-containing aquifers and groundwater.

Thus, a simulated aquifer sediment system and groundwater microcosms were used to monitor the survival of G4 by: In Situ Bioremediation of Trichloroethylene. Becki Tlusty. Introduction.

Volatile organic compounds (VOC) are present at over two-thirds of Superfund, Resource Conservation and Recovery Act of (RCRA), and U.S. Department of Defense sites (EPA ). Thus, remediation of VOCs is a top concern to the Environmental Protection Agency.

Burkholderia cepacia G4 PR1 is a Tn5 insertion mutant which constitutively expresses a toluene ortho-monooxygenase that degrades trichloroethylene (TCE).

McCarty PL, Hopkins GD, Munakata-Marr J, Matheson VG, Dolan ME, Dion LB, Shields M, Forney LJ, Tiedje JM. Bioaugmentation with Burkholderia cepacia PR1 for the in situ bioremediation of trichloroethylene contaminated groundwater.

Cited by: 1. In situ bioremediation of trichloroethylene using Burkholderia cepacia G4 PR1: analysis of microbial ecology parameters for risk assessment / Richard A.

Author: Richard A. Snyder. The effect of biomass density, effect of different ratios of trichloroethylene (TCE) and toluene on biomass, and strategies to cope with problems using acclimated Burkholderia cepacia G4 were investigated.

Complete degradation of TCE was achieved when B. cepacia G4 was exposed to mg/L TCE and 10 mg/L toluene after starvation of h. Overall results of this study show that degradation Cited by: 3. Cytotoxicity Associated with Trichloroethylene Oxidation in Burkholderia cepacia G4 Article (PDF Available) in Applied and Environmental Microbiology 67(5) June with 37 Reads.

21 Genetically Engineered Microorganisms and Bioremediation Pseudomonas putida F1/pSMMO20 Burkholderia cepacia G4 PR1 Ralstonia eutropha AEK/pYK 2,4-Dichlorophenoxyacetic acid (2,4-D) Pseudomonas putida PPO(pRO) and PPO(pRO) Pseudomonas cepacia RHJ1. Degradation of trichloroethylene by Pseudomonas cepacia G4 and the constitutive mutant strain G4 PR1 in aquifer microcosms.

Krumme ML, Timmis KN, Dwyer DF Appl Environ Microbiol, 59(8), 01 Aug Keywords: trichloroethylene, electrochemical treatment, in-situ decomposition, contamination of soil, electroosmosis 1. Introduction Trichloroethylene (TCE) has an excellent decreasing capacity, so it is often used as a solvent for dry cleaning, and is still used.

Abstract. Trichloroethylene (TCE), a chlorinated organic solvent, is one of the most common and widespread groundwater contaminants worldwide. Among the group of TCE-degrading aerobic bacteria, Burkholderia cepacia G4 is the best-known representative.

This strain requires the addition of specific substrates, including toluene, phenol, and benzene, to induce the enzymes to degrade by: 6. Therefore, in situ methods to determine both the rate and extent of dechlorination are needed to assess the potential for intrinsic bioremediation, as well as to design and monitor engineered bioremediation projects.

The methods often used to study dechlorination processes do not yield sufficient data to evaluate the rate and extent of degradation. Trichloroethylene (TCE) was removed from soils by using a wheat rhizosphere established by coating seeds with a recombinant, TCE-degrading Pseudomonas fluorescens strain that expresses the tomA + (toluene o-monooxygenase) genes from Burkholderia cepacia PR1 23 (TOM 23C).A transposon integration vector was used to insert tomA + into the chromosome of P.

fluorescensproducing a Cited by:. JOURNAL OF BIOSCIENCE AND BIOENGINEERING Vol. 92, No. 1, REVIEW Current Bioremediation Practice and Perspective TOMOTADA IWAMOTO' AND MASAO NASUZ* Department of Bacteriology; Kobe Institute of Health, Minatojima-nakamachi, Chuo-ku, Kobe ' and Environmental Science and Microbiology; Graduate School ofPharmaceutical Sciences, Osaka Cited by: Aerobic trichloroethylene (TCE) biodegradation was investigated in small-column aquifer microcosms repeatedly fed solutions containing mg/L phenol or 15 mg/L lactate and μg/L TCE every two to three days.

The effectiveness of TCE cometabolism by an indigenous phenol-fed microbial population declined significantly during a day by:   To examine the trichloroethylene (C2HCl3)-degrading capability of five microorganisms, the maximum rate, extent, and degree of C2HCl3 mineralization were evaluated for Pseudomonas cepacia G4, Pseudomonas cepacia G4 PR1, Pseudomonas mendocina KR1, Pseudomonas putida F1, and Methylosinus trichosporium OB3b using growth conditions commonly reported in the literature for Cited by: